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May 2, 2026SHILAP Revista de lepidopterologíaOpen Access

Integrated characterization of six sesquiterpene synthases unravels diversified terpene biosynthesis in the mint species Leucosceptrum canum

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Authors

QSQinqin ShenSCShaojun ChengCZChenxiao Zhao

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Overview

Randomized trial shows mechanistic insights into terpene biosynthesis in Leucosceptrum canum, indicating potential for sustainable production of sesquiterpenoids.

Key Points

  • The study aims to characterize six sesquiterpene synthases from Leucosceptrum canum to understand their functional diversity and biosynthetic mechanisms.
  • Functionally characterized six sesquiTPSs through heterologous expression in Escherichia coli.
  • Utilized GC-MS and NMR for product identification, alongside phylogenetic analysis and gene expression profiling.
  • Applied metabolic engineering strategies to establish high-yield production systems for the targeted sesquiterpenoids.
  • Generated various sesquiterpenoids including germacrene A and (+)-β-himachalene from LcTPS3-LcTPS8.
  • Phylogenetic analysis revealed evolutionary divergence with classification into TPS-a and TPS-b subfamilies.
  • Established production systems achieving up to 618.73 mg L⁻¹ of six sesquiterpenoids.

Cite This Study

Shen et al. (2026) studied this question.

synapsesocial.com/papers/69f593f271405d493affec5bhttps://doi.org/10.3389/fpls.2026.1833733
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