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May 2, 20260 citations

Detection of Helicobacter Pylori and Resistance Genes Using Multiplex Fluorescent PCR

Multiplex fluorescent PCR-based detection of Helicobacter pylori nucleic acid and antibiotic resistance genes in clinical stool samples: a performance evaluation from Gansu Province, China.

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Authors

YDYeze DongJYJinxia YangBXBaoyuan Xie

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Overview

Randomized trial evaluates H. pylori detection and resistance in clinical stool samples, suggesting a reliable diagnostic tool for limited-resource settings.

Key Points

  • Evaluate the clinical performance of a multiplex fluorescent PCR kit for detecting H. pylori and antibiotic resistance in stool samples.
  • Enrolled 535 patients with suspected H. pylori infection.
  • Tested stool samples using multiplex fluorescent PCR for H. pylori DNA and resistance mutations.
  • Compared results against gastric biopsies cultured for H. pylori and antimicrobial susceptibility testing.
  • Culture confirmed H. pylori in 383/535 patients (71.6%).
  • MFQ-PCR showed 99.5% sensitivity (95% CI: 98.3-99.9%) and 94.7% specificity (95% CI: 90.5-97.6%).
  • Resistance rates were high: metronidazole 92.0%, levofloxacin 51.3%, clarithromycin 51.0%.

Cite This Study

Dong et al. (2026) studied this question.

synapsesocial.com/papers/69f593f271405d493affecf9https://doi.org/10.1186/s12879-026-13476-3
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