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May 2, 20261 citations

Native Aquatic Plastispheres in a River-Wastewater Catchment: Carbapenem-Resistant Bacteria Isolation and Microscopy-Based Structural Analysis.

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RDRolbiecki DamianJKJaszczyszyn KatarzynaWSWawrocki Sebastian

Key Points

  • This research focuses on isolating carbapenem-resistant bacteria in plastispheres and understanding their ecological implications.
  • Used microscopy to analyze bacterial clusters on plastic surfaces in a river-wastewater catchment.
  • Isolated bacteria to study the mechanisms of antimicrobial resistance (AMR) in these microenvironments.
  • Investigated spatially resolved microbial community structures related to AMR dissemination.
  • Identified dense clusters of bacteria on plastic surfaces, indicating potential hotspots for horizontal gene transfer.
  • Showed that plastispheres serve as vectors for antimicrobial resistance, contributing to its persistence in aquatic environments.
  • Highlighted the need for integrating genomic data to understand resistance gene mobility in these systems.

Abstract

), and Pseudomonas putida (efflux-based resistance). Microscopy revealed dense bacterial clusters on plastic surfaces, suggesting microenvironments that may facilitate cell-cell interactions, including horizontal gene transfer. These findings highlight plastispheres not only as vectors of AMR but also as potential evolutionary hotspots shaping resistance persistence and dissemination in aquatic systems. Future integrating metagenomic and genomic data on resistance gene mobility with spatially resolved microbial community structure will provide critical insights into the mechanisms and risks of AMR dissemination in plastisphere environments.

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Cite This Study

Damian et al. (2026) studied this question.

synapsesocial.com/papers/69f5943c71405d493affeff7https://doi.org/10.1111/1462-2920.70312
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