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May 2, 20260 citations

Contamination potential of non-resorbable membranes intended for exposure in the oral cavity during regenerative procedures - In vitro study.

LSLucas Trevisan SuzzinAWArtur Villas Bôas WeckwerthVOVerci Alves de Oliveira

Key Points

  • This study aims to evaluate microbial contamination in non-resorbable membranes used in oral regenerative procedures.
  • In vitro study with fifty-six samples divided into control and contaminated groups.
  • Microbial analysis was performed by counting colony-forming units and using advanced imaging techniques.
  • Statistical comparisons were made using one-way ANOVA and Tukey's tests, with significance set at 5%.
  • Membrane BH had the lowest contamination level, while membrane L exhibited the highest (p = 0.005).
  • All membranes became contaminated, with variations based on surface characteristics and design.
  • Surface morphology influenced the contamination patterns noted among the different membranes.

Abstract

BACKGROUND: Some non-resorbable membranes are indicated to be left exposed in the oral cavity during regeneration procedures. Given the frequency or indication of exposure of these membranes in the oral cavity, it is worth studying their pattern of contamination, which may lead to undesirable clinical results. PURPOSE: This study aimed to evaluate and compare microbial contamination among non-resorbable membranes intended for exposure in the oral cavity during regenerative procedures. METHODS: (STS). Fifty-six samples were divided into groups: group S (control) - sterile membranes; group C - membranes contaminated with human oral biofilm. These samples were analyzed by counting colony-forming units and scanning electron microscopy/energy dispersive spectroscopy. One-way ANOVA and Tukey's tests were used to compare colony-forming units among the contaminated groups. The paired t-test was used to compare the colony-forming units between the two groups of each membrane, with a significance level of 5%. RESULTS: The membranes showed similar contamination values, with the BH membrane being the least contaminated and the L membrane the most contaminated (p = 0.005). The membranes presented surfaces with different patterns and designs, also showing different levels of contamination that seem to be related to surface characteristics and composition. CONCLUSION: All studied membranes intended for exposure in the oral cavity became contaminated, showing different patterns, with membrane BH exhibiting the lowest contamination.

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Cite This Study

Suzzin et al. (2026) studied this question.

synapsesocial.com/papers/69f5945c71405d493afff28dhttps://doi.org/10.1186/s12903-026-08181-1
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