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May 3, 20260 citations

Identification of multiple arbovirus infections/exposure in Northeastern Brazil using a multiplex microsphere immunoassay.

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WWWei-Kung WangENEduardo Martins NettoSHSzu‐Chia Hsieh

Key Points

  • The aim is to identify multiple arbovirus infections in participants using a multiplex immunoassay.
  • Evaluated sensitivity and specificity of multiplex IgG immunoassay in 374 individuals.
  • Assessed reactions to NS1 proteins for ZIKV and YFV.
  • Implemented algorithms and confirmatory tests to identify co-infections.
  • Reported high sensitivity of NS1-based IgG MIA, ranging from 94.4% to 100% for most viruses.
  • Overall low specificity observed for ZIKV and WNV NS1 due to cross-reactivities.
  • Identified multiple infections, including DENV + ZIKV and others, in the majority of participants.

Abstract

= 374) to evaluate the sensitivity/specificity of our multiplex IgG MIA. We reported high sensitivity of nonstructural protein 1 (NS1)-based IgG MIA (94.4%-100% except YFV NS1) and overall low specificity of ZIKV and WNV NS1 due to cross-reactivities from repeated orthoflavivirus infections, including secondary DENV and DENV + ZIKV infections. Using our algorithm and confirmatory tests, we reported multiple arbovirus infections or exposure (DENV + ZIKV, DENV + YFV, DENV + ZIKV + YFV, and/or CHIKV) in the majority of participants from a town in Northeastern Brazil.

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Cite This Study

Wang et al. (2026) studied this question.

synapsesocial.com/papers/69f6e5308071d4f1bdfc5e97https://doi.org/10.1128/jcm.00013-26
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