A fluorescence-activated droplet sorting assay for ultra-high-throughput screening of PET hydrolases based on a pH indicator.

Key Points

• This research aims to develop a pH-based fluorescence-activated droplet sorting assay for screening PET hydrolases efficiently.
• Integration of a pH indicator in the fluorescence-activated droplet sorting assay.
• Assessment of the assay's performance in ultra-high-throughput conditions.
• Evaluation of PET hydrolase activity through fluorescence detection.
• The pH-based assay successfully identifies functional PET hydrolases with high throughput.
• Efficiency metrics indicate superior performance compared to conventional screening methods.

Abstract