Detailed Protocol for Expansion of Large Tissue Samples for Expansion Microscopy.

Key Points

• To present an optimized protocol for expansion microscopy to enhance the study of tissue samples.
• Developed a protocol for expansion microscopy specifically for 2 mm diameter and 1 mm thickness tissue biopsies from rat inner medulla kidneys.
• Evaluated the physical enlargement and transparency of biological samples using expansion microscopy techniques.
• Suggested minor modifications for application on other tissue types.
• Demonstrated enhanced optical resolution by physically enlarging samples, increasing distances between fluorophores.
• Achieved successful enlargement of inner medulla kidney samples, allowing for clearer imaging.
• Provided a viable protocol adaptation for various tissue types beyond original kidney samples.