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May 6, 2026Pets0 citationsOpen Access

A Novel Postbiotic Improves Stool Consistency in Dogs: A Randomized, Double-Blind Placebo-Controlled Study

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ASAylesse SordilloJHJonna HeldrichRTRaphaël Turcotte

Key Points

  • To evaluate the effects of a canine immune health postbiotic on stool consistency and gut microbiome in dogs with loose stools.
  • Conducted a randomized, double-blind, placebo-controlled trial with colony-housed dogs.
  • Twenty dogs with consistent loose stools received either CIHP or placebo for 28 days mixed with their diet.
  • Measured stool consistency and fecal biomarkers at multiple time points using the Waltham fecal score.
  • CIHP improved stool quality compared to placebo (p-value = 0.03).
  • Microbiome profiling revealed 16 taxa significantly shifted with CIHP versus 1 with placebo.
  • Fecal biomarkers remained stable in both groups within reference ranges, indicating normal physiological responses.

Abstract

Postbiotics are an emerging ingredient class which have promising potential to support canine gut function by delivering beneficial microbial compounds directly to the gut. We tested a canine immune health postbiotic (CIHP) in a randomized, double-blind, placebo-controlled study of twenty colony-housed dogs (ten per group) selected for having consistently loose stools but with no diagnosed gastrointestinal disease. After a 5-day wash-in and 5-day baseline, dogs received 12 mg/kg body weight per day of CIHP or a placebo for 28 days mixed with their normal dry diet. The primary outcome was stool consistency (Waltham fecal score), measured on Days 0, 14, and 28; secondary outcomes included fecal gut-health biomarkers and fecal microbiome composition from 16S rRNA sequencing, measured on Days 0 and 28. CIHP improved stool quality (p-value = 0.03), while placebo did not (p-value = 0.5), and CIHP showed a trend toward increasing the odds of individual dogs showing improved fecal scores by Day 28 compared to placebo (p-value = 0.07). Microbiome profiling revealed broader community remodeling with CIHP than the placebo (16 taxa significantly shifted with CIHP vs. 1 with the placebo), consistent with stool quality being impacted by both gastrointestinal and gut microbiome functions. Fecal biomarkers that reflect gut health (pH, dry matter, short-chain fatty acids, dysbiosis index, calprotectin) were within reference ranges at baseline and remained stable in both groups, indicating benefits occurred within a normal physiological window. Together, these findings show that CIHP can improve stool consistency while reshaping the gut microbiome in otherwise healthy dogs prone to loose stools. Future studies in home-environment dogs across breeds, ages, and living conditions are needed to generalize these findings to the broader canine population.

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Cite This Study

Sordillo et al. (2026) studied this question.

synapsesocial.com/papers/69fa8e8904f884e66b530db5https://doi.org/10.3390/pets3020019
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