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May 6, 2026Diagnostics0 citationsOpen Access

Meat-Borne Bacterial Pathogen Detection: Conventional, Molecular and Emerging AI-Based Strategies

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AHAthar HussainQAQindeel AbbasMNMuhammad Nadeem

Key Points

  • To synthesize advancements in detecting meat-borne bacterial pathogens using molecular and AI strategies.
  • Review of current detection techniques in meat, focusing on AI applications.
  • Evaluation of major foodborne pathogens in the meat supply chain.
  • Analysis of key genomic databases for AI model training.
  • Identification of major pathogens including Salmonella, Escherichia coli, and others.
  • Demonstration of AI systems achieving 100% bacterial capture capability.
  • Assessment of biosensors and CRISPR-based assays enhancing detection accuracy.

Abstract

Meat serves as a prime medium for the growth of foodborne pathogens due to its rich protein content and high water activity, contributing significantly to the global burden of foodborne illnesses. This review synthesizes current advances in meat-borne bacterial pathogen detection with particular emphasis on emerging artificial intelligence (AI)-enabled applications. Major pathogens of concern, including Salmonella, Listeria monocytogenes, Escherichia coli, Campylobacter, Clostridium, and Staphylococcus aureus, are examined in relation to their relevance across the meat supply chain. Recent progress in biosensors (clustered regularly interspaced short palindromic repeats), CRISPR-based assays, isothermal amplification, and metagenomics is evaluated alongside the growing role of AI in automating signal interpretation, enhancing image-based diagnostics, and supporting early contamination prediction. AI-based systems have proved 96.4–104% recovery and 100% bacterial capture ability. Embedding AI methods in a wet lab demands technical and logical modeling, as well as learning and calibration decorum. Nonetheless, AI readiness and full-scale application for meat-borne pathogens surveillance are on the way. Furthermore, additional focus is aligned on meat-borne bacterial pathogen genomic databases, i.e., (NCBI Pathogen Detection, EnteroBase, VFDB, ComBase, and GenBank), which serve as critical training resources for AI models for outbreak tracking, virulence profiling, and antimicrobial resistance (AMR) prediction. By integrating molecular methods, genomic surveillance, and AI-driven analytics, this review presents a framework for strengthening meat safety systems. This will improve early detection capabilities and support data-driven public health interventions in the future.

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Cite This Study

Hussain et al. (2026) studied this question.

synapsesocial.com/papers/69fa986a04f884e66b53217ehttps://doi.org/10.3390/diagnostics16091360
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