A guide to selecting high-performing antibodies for SCARB2 (UniProt ID: Q14108) for use in western blot, immunoprecipitation, and immunofluorescence

Scavenger receptor class B member 2 (SCARB2), also known as lysosomal integral membrane protein type 2 (LIMP-2), is a multifunctional glycoprotein critical for lysosomal biogenesis and neuronal homeostasis. Here we have characterized twelve SCARB2 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.