Modeling the Short-Term Response to Nitrogen that Coordinates Events in Lateral Root Initiation

Nitrogen (N) is an essential macronutrient and its bioavailability plays a major role in tuning plant development to the environmental nutrient status.To find novel factors in early root system architecture responses to N conditions, we performed Arabidopsis thaliana root transcriptome profiling of a short-term time course in limiting and sufficient N conditions.Using this data, we inferred transcriptional regulatory networks in each condition, which revealed the N-condition specific jasmonate responses.We found transcriptional factor (TF) ERF107 plays a generalized role in lateral root development while LBD13 is specific to N-limiting conditions.Further, we used a lateral root single-cell-identity specific transcriptome dataset to model the roles of TFs in early stages of lateral root development.We linked the N time course transcriptomics, lateral root mutant phenotypes, and cell-type specific profiling approaches to find and test the roles of TFs that are involved in early root patterning responses to N conditions.