, SRS imaging revealed a significant accumulation of nystatin in the fungal membrane and the internalization of the drug. Spectral phasor analysis was applied to identify the discrete nystatin signal from the cellular content using hyperspectral SRS imaging. This work provides the first label-free and direct visualization of nystatin binding in biological membranes and the subsequent trafficking in fungal cells using SRS microscopy.
Tipping et al. (Mon,) studied this question.