) cell lysates. However, the methylation of substrates with more than one nucleophilic atom requires sophisticated analytical tools for accurate monitoring of the regiospecificity of methyltransferases. This complexity often limits the broader exploitation of methyltransferases, particularly in enzyme engineering efforts, where large datasets are generated and must be efficiently analyzed. This review critically examines the high-throughput screening assays developed to date for methyltransferase activity.
Fotiadou et al. (2026) studied this question.