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May 7, 2026Journal of Neurology Research0 citationsOpen Access

Genotype-Guided Dual Antiplatelet Therapy in CYP2C19 Loss-of-Function Carriers With Stroke or Transient Ischemic Attack: A Meta-Analysis of Ticagrelor–Aspirin Versus Clopidogrel–Aspirin

IWI Nyoman WindianaMIMuhammad IqhrammullahLKLuh Putu Lina Kamelia

Key Result

Ticagrelor combined with aspirin significantly reduced the risk of recurrent stroke at 90 days (RR 0.72) compared with clopidogrel-aspirin in CYP2C19 loss-of-function carriers with TIA or minor stroke.

Key Points

  • This research aims to assess the effectiveness and safety of ticagrelor plus aspirin compared to clopidogrel plus aspirin for individuals with CYP2C19 loss-of-function variants suffering from stroke or TIA.
  • Conducted a systematic review and meta-analysis following PRISMA guidelines.
  • Screened electronic databases like PubMed and Scopus for randomized trials comparing ticagrelor–aspirin and clopidogrel–aspirin in CYP2C19 LOF carriers.
  • Evaluated outcomes including recurrent stroke, composite vascular events, and mortality.
  • Ticagrelor–aspirin significantly reduced the risk of recurrent stroke at 90 days compared to clopidogrel–aspirin (P < 0.00001).
  • Showed a lower incidence of composite vascular events with ticagrelor–aspirin (P < 0.00001).
  • Reduced intracranial hemorrhage incidence (P = 0.005) and mortality (P < 0.0001) with ticagrelor-based therapy.

Study Design

Type

Meta-Analysis (n=64,128)

Structured PICO

Does dual antiplatelet therapy with ticagrelor plus aspirin reduce recurrent stroke and vascular events compared to clopidogrel plus aspirin in CYP2C19 loss-of-function carriers with TIA or minor ischemic stroke?

P
Population
64,128 adult patients (18-80 years) with minor ischemic stroke or transient ischemic attack (TIA) who are confirmed carriers of CYP2C19 loss-of-function (LOF) alleles, pooled from 12 randomized controlled trials.
I
Intervention
Ticagrelor plus aspirin (typical regimen across included trials: ticagrelor 180 mg loading dose on day 1 followed by 90 mg twice daily for days 2-90, plus aspirin 75-300 mg loading dose on day 1 followed by 75-100 mg daily for 21 days).
C
Comparator
Clopidogrel plus aspirin (typical regimen across included trials: clopidogrel 300 mg loading dose followed by 75 mg daily for days 2-90, plus aspirin 75-300 mg loading dose on day 1 followed by 75-100 mg daily for 21 days).
O
Outcome
Incidence of recurrent ischemic or hemorrhagic stroke within 90 days.hard clinical

In CYP2C19 loss-of-function carriers with TIA or minor ischemic stroke, genotype-guided dual antiplatelet therapy with ticagrelor and aspirin significantly reduces recurrent stroke, composite vascular events, and mortality compared to clopidogrel and aspirin.

Main Result

Effect estimate: RR 0.72 (95% CI 0.65-0.80)

p-value: p=<0.00001

Limitations

  • Variability in study design, sample size, baseline patient characteristics, and follow-up durations across the included trials
  • Ticagrelor remains associated with an increased bleeding tendency in broader clinical practice
  • Lack of sex-stratified data in the included studies precluded subgroup analyses based on gender
  • Lack of detailed reporting on cause-specific mortality (neurological versus non-neurological)
  • Absence of patient-level data restricted more granular analyses, including stratification by stroke subtype
  • Variability in study design, sample size, baseline patient characteristics, and follow-up durations across included trials
  • Lack of sex-stratified data precluding subgroup analyses based on gender
  • Absence of patient-level data restricting granular analyses by stroke subtype

Abstract

Background: Individuals presenting with transient ischemic attack (TIA) or minor ischemic stroke face a substantial risk of early stroke recurrence. Although dual antiplatelet therapy (DAPT) lowers the incidence of ischemic events, it is accompanied by an elevated bleeding risk. Moreover, CYP2C19 genetic polymorphisms, particularly loss-of-function (LOF) variants, may impair clopidogrel metabolism and attenuate its antiplatelet efficacy, underscoring the need to evaluate alternative therapeutic strategies. This study aimed to compare the efficacy and safety of ticagrelor plus aspirin versus clopidogrel plus aspirin among CYP2C19 LOF carriers with TIA or minor ischemic stroke. Methods: A systematic review and meta-analysis was performed following PRISMA recommendations. Electronic databases including PubMed, Scopus, and Google Scholar were systematically screened to identify randomized controlled trials evaluating ticagrelor–aspirin versus clopidogrel–aspirin in patients harboring CYP2C19 LOF alleles. Primary and secondary outcomes comprised 90-day recurrent ischemic or hemorrhagic stroke, composite vascular events, intracranial hemorrhage, and all-cause mortality. Pooled risk ratios (RRs) with corresponding 95% confidence intervals (CIs) were calculated to estimate treatment effects. Results: Twelve eligible studies encompassing 64,128 CYP2C19 LOF carriers were included in the final analysis. Compared with clopidogrel–aspirin therapy, ticagrelor–aspirin was associated with a significantly lower risk of recurrent stroke at 90 days (P < 0.00001) and composite vascular events (P < 0.00001). Additionally, ticagrelor-based DAPT demonstrated a reduced incidence of intracranial hemorrhage (P = 0.005) and mortality (P < 0.0001). Conclusion: Among CYP2C19 LOF carriers with TIA or minor ischemic stroke, ticagrelor combined with aspirin appears to offer superior protection against recurrent stroke and vascular complications compared with clopidogrel–aspirin, while also reducing intracranial hemorrhage and mortality. Nevertheless, individualized risk–benefit assessment remains essential, particularly considering the bleeding profile associated with ticagrelor.

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Cite This Study

Windiana et al. (2026) conducted a meta-analysis in Transient ischemic attack (TIA) or minor ischemic stroke with CYP2C19 loss-of-function alleles (n=64,128). Ticagrelor plus aspirin vs. Clopidogrel plus aspirin was evaluated on Recurrent ischemic or hemorrhagic stroke within 90 days (RR 0.72, 95% CI 0.65-0.80, p=<0.00001). Ticagrelor combined with aspirin significantly reduced the risk of recurrent stroke at 90 days (RR 0.72) compared with clopidogrel-aspirin in CYP2C19 loss-of-function carriers with TIA or minor stroke.

synapsesocial.com/papers/69fc2c1f8b49bacb8b347b51https://doi.org/10.14740/jnr1101
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Ticagrelor Aspirin vs Clopidogrel Aspirin in <i>CYP2C19</i> Loss-of-Function Carriers With Minor Stroke or TIA Stratified by Risk Profile2022 · 17 citations
  2. 2Genotype-guided ticagrelor/prasugrel versus clopidogrel therapy in stroke patients with <i>CYP2C19</i> loss of function alleles: a systematic review and meta-analysis2025 · 1 citations
  3. 3Comparison between clopidogrel and ticagrelor in CYP2C19 loss-of-function alleles coronary artery disease and stroke patients: a meta-analysis2025 · 5 citations
  4. 4Ticagrelor plus aspirin in patients with minor ischemic stroke and transient ischemic attack: a network meta-analysis2023 · 7 citations
  5. 5Time Course for Benefit and Risk With Ticagrelor and Aspirin in Individuals With Acute Ischemic Stroke or Transient Ischemic Attack Who Carry <i>CYP2C19</i> Loss-of-Function Alleles2022 · 28 citations