Protective effect of Platycodon grandiflorus extract on LPS-induced cytotoxicity against HT-29 cells

Key Points

• The aim is to investigate the protective effects of Platycodon grandiflorus extract against LPS-induced cytotoxicity in HT-29 cells.
• In vitro analysis of HT-29 cells exposed to LPS and treated with Platycodon grandiflorus extract.
• Assessment of cell viability, oxidative stress, and inflammation markers (TLR4, MyD88, p-NF-κB, iNOS).
• Measurement of tight junction proteins (ZO-1, occludin, claudin-1) and apoptosis-related factors (p-AKT, p-JNK, BAX, Bcl-2) using HPLC.
• Platycodon grandiflorus extract increased cell viability and reduced oxidative stress in HT-29 cells exposed to LPS.
• Expression of inflammatory markers TLR4, MyD88, and iNOS was significantly decreased, while tight junction proteins showed increased levels.
• HPLC analysis confirmed the presence of platycodin D as a major bioactive compound.

Abstract