Development of a Colourimetric Loop‐Mediated Isothermal Amplification Assay for Rapid Detection of Streptococcus dysgalactiae

Key Points

• This research aims to create a simple and effective method to detect the bacteria Streptococcus dysgalactiae in aquatic environments.
• Developed a colourimetric loop-mediated isothermal amplification (LAMP) assay.
• Tested under isothermal conditions to ensure accuracy.
• Evaluated specificity against eight non-target bacterial species.
• Achieved high specificity with no cross-reactivity to non-target species.
• Provided clear visual readouts for positive detections.
• Showed potential for field-based testing in aquaculture settings.

Abstract