Study of Cyclophilin A Polymorphism in Women Patients with Cytomegalovirus Infections

Abstract Background: Cyclophilin A (CypA) is a highly conserved protein that is expressed in a wide range of tissues and cell types and plays roles in inflammation, oxidative stress, and viral infection. CypA may influence oxidative stress pathways during cytomegalovirus (CMV) infection through its modulation of cellular redox balance and signaling pathways involved in oxidative stress responses; CypA is a promising marker of abortion in women infected with CMV. Aims: To investigate CypA genetic polymorphism and its serum in Iraqi women with abortion due to infection with CMV. Methods and Materials: A total of 200 blood samples were collected from women who had abortions and were admitted to the Maternity and Children’s Hospital in Hilla. In total, 50 healthy women control were diagnosed with VIDAS machine for IgM CMV and IgG CMV kits, single nucleotide polymorphism sites of rs4720485 and rs8177826 were analyzed by PCR-RFLP, and CypA serum level determined by enzyme-linked Immunosorbent assay (ELISA). Results: Among the total number of samples, we found 60 (30%) CMV were positively obtained from women with abortion, divided into six (3%) samples that were seropositive to CMV IgM, 4(2%) samples that were seropositive to CMV IgM + IgG and 50 (25%) samples positive to IgG only, ELISA analysis showed CypA level concertation with CMV were IgM and IgG are high, analysis for PPIA polymorphisms (rs 4720485A/T and rs 8177826C/G) that both are not significant between the patient group and control group. Conclusions: To evaluate the role of CypA levels in women who experienced abortion due to CMV infections during pregnancy. The results of the study demonstrated a significant increase in CypA levels in women who were infected with CMV, leading to abortion.