Target-to-Signal Conversion and Spatial Enrichment Cascade Boosts CRISPR/Cas12a Biosensing for Trace-Level Pathogen Detection

Key Points

• This research aims to improve pathogen detection methods using CRISPR/Cas12a technology.
• Implemented target-to-signal conversion techniques to enhance detection sensitivity.
• Used a spatial enrichment cascade to optimize the biosensing process.
• Conducted trials using samples contaminated with typhimurium.
• Achieved a significant increase in detection sensitivity compared to traditional methods.
• Successfully detected typhimurium at trace levels, indicating enhanced biosensing capabilities.
• Results suggest improved safety in food testing environments.

Abstract