High Level of Matrix Metaloproteinase-9 and Toll-Like Receptor-4 in Gingival Cervicular Fluid of Periodontitis Patients

Abstract Background: Periodontitis is a prolonged inflammatory issue that destroys the connective tissue and bone that hold the teeth in place. This illness is regarded as one of the leading causes of tooth deterioration. Poor dental health can have a significant impact on overall health, pain, eating, chewing, smiling, and communicating. Furthermore, missing teeth have a substantial impact on people’s quality of life and overall well-being. Severe periodontitis, which affects 5%–20% of the adult population worldwide, can result in tooth loss. Objectives: This study sought to determine the levels of matrix metaloproteinase-9 (MMP9) and toll-like receptor-4 (TLR4) in periodontitis patients’ gingival cell fluid and how these levels connected to clinical attachment loss and probing pocket depth. Materials and Methods: Ninety samples were used in this study, including 60 samples; 38 of men and 22 of women who suffer from periodontitis, whereas 11 of men and 19 of women were considered as the control group. This study extended from December 2022 to February 2023. The age of patients and control were ranging from 30 to 63 years old. The samples were analyzed by the enzyme-linked immunosorbent assay testes technique to measuring level of MMP9 and TLR4. Results: The level of TLR4 and MMP9 was higher in patients than control concentrations (562.45 ± 70.06, 77.12 ± 11.51 pg/mL) and (8.07 ± 0.19, 6.36 ± 0.51 pg/mL), respectively. Conclusion: According to investigation, patients with periodontitis had a greater amount of MMP9 and TLR4 than healthy people.