Investigating the Impact of miR-21 rs1292037:T>C, miR-449b rs10061133:A>G and PDCD4 rs6585018:G>A Haplotype Polymorphism in Iraqi Breast Cancer Patients

Abstract Background: Breast cancer is considered a hereditary disease characterized by the presence of a large number of genetic changes that have a different effect on its pathogenicity. Objective: The present study intended to assess the potential association between micro-ribonucleic acid (miRNA) and PDCD4 single nucleotide polymorphisms (SNPs) map to different genomic regions (rs6585018:G>A, rs1292037:T>C, and rs10061133:A>G) with breast cancer in Iraqi patients. Materials and Methods: A total of 180 peripheral blood samples were collected from 120 breast cancer patients and 60 healthy women as a control group (age range 15–80 years). Following genomic DNA extraction and polymerase chain reaction (PCR) amplification of sequence of interest, PCR products of the participants were genotyped using TaqMan fluorescent oligonucleotide for SNP analysis. Results: Significant association was identified for the assessed SNPs with breast cancer. This was evident when the allelic and genotypic patterns T, TT, A, AA and A, AA map to miR-21 rs1292037:T>C, miR-449b rs10061133:A>G, PDCD4 rs6585018:G>A, respectively seemed to confer breast cancer risk factors in the studied set of patients. While breast cancer protective potential was exhibited for the C, CC, G, GG and G, GG genetic patterns located at the aforementioned genomic regions. Additionally, haplotype analysis of the assessed miRNAs and PDCD4 genomic regions (map to 17q23.2, 5q11.2, and 10q25.2) suggest that the three chromosomal regions are associated with breast cancer, where the GCA haplotype pattern confers a risk factor, and GCG retains a protective potential of the disease ( P = 0.0038). Conclusions: Overall, the present study findings suggest that breast cancer pathogenicity is significantly influenced by the association between the miR-21 rs1292037:T>C, miR-449b rs10061133:A>G, and PDCD4 rs6585018:G>A SNPs haplotype.