The Ability for the Extracellular Production of Iron Oxide Nanoparticles by Klebsiella pneumoniae and Staphylococcus aureus

Abstract Background: The biological production of nanoparticles is emerging as a possible method for nanoparticle synthesis due to its simplicity and lack of toxicity. Recent research has looked into microorganisms as a potential biofactor for the manufacture of many nanoparticles (NPs). Objectives: The aim of this paper is to screen a variety of common bacteria in order to assess the extracellular biosynthetic potential of metallic NPs like iron oxide (Fe 2 O 3 ) NPs Materials and Methods: By using Staphylococcus aureus and Klebsiella pneumonia as possible candidates for the quick synthesis of NPs, ferric nitrate was exploited as a source of Fe 2 O 3 NPs. A reduction of aqueous solutions with bacterial cell-free filtrates was used to create Fe 2 O 3 NPs. Results: Characterization of synthesized NP conducted by UV–Vis spectroscopy and the maximum peak at 407 nm absorbance for K. pneumoniae Fe 2 O 3 NPs. Also, 408 nm S. aureus Fe 2 O 3 NPs. Conclusion: The ability of the isolated bacteria to synthesize iron oxide NPs in large quantities is demonstrated by the success of the stabilized F e 3O 4 NP synthesis, which was capped by the organic group.