In Vitro Study the Effects of Amphotericin B Silver Nanoparticles and Amphotericin B on the Leishmania tropica

Abstract Background: Iraq is an endemic country with a high frequency of both types of leishmaniasis, visceral and cutaneous, with several clinical manifestations. However, multidrug-resistant strains are serious problems for public health worldwide. So, the continuous development of antiparasitic agents has become of increasing importance for medicine. Objectives: This study aimed to estimate the inhibitory activity of amphotericin B silver nanoparticles against Leishmania tropica in comparison to that of amphotericin B in vitro . Materials and Methods: Leishmania tropica isolate samples provided by Al-Nahrain University’s Biotechnology Research Center. The stock culture of L. tropica used in this study has been maintained in the laboratory by weekly serial subculturing in RPMI 1640 medium. Results: The present study revealed that the final test of transmission electron microscopy analysis revealed that the infection with L. tropica has more effects when treated (Amph-B-AgNPs), and the parasite was well distributed and most compounds were outside parasites compared with the parasite before treatment. When the concentration of Amph-B-AgNPs increased, the antiparasitic effect increased while the parasite’s viability curve decreased. Conclusions: The study showed that the amphotericin B silver nanoparticles had the best therapeutic effects on L. tropica compared with amphotericin B alone.