The Gene Expression of TNF-α Predicting ICSI Outcome

Abstract Background: According to the updated international glossary on infertility and fertility care, infertility is defined as a disease characterized by the inability to achieve a clinical pregnancy after twelve months of regular, unprotected sex. Millions of people are affected by infertility, which also affects their families and communities. Objective: The aim of the current study was to assess the association gene expression of tumor necrosis factor alpha (TNF-α) with implantation success in women undergoing intracytoplasmic sperm injection. Materials and Methods: The current study examined 150 infertile women who received intracytoplasmic sperm injection at the Tiba Center for Infertility, Babil, Iraq and Al-Kafeel Hospital, Karbala, between May 2022 and November 2022. Women with positive biochemical pregnancies were included in the first study group, and those with negative pregnancies were included in the second. Blood samples were taken from individuals for the study. The gene expression of TNF-α was examined. Results: The median and interquartile range of TNF-α in positive and negative pregnancy were 17.13 (27.58) and 4.23 (27.69), respectively, and the range was from 0.00 to 759.78 and 0.00 to 120.99, respectively. The difference in mean rank was statistically significant ( P = < 0.001). Conclusion: Gene expression of TNF-α can affect endometrial thickness, leading to implantation failure.