TAGLN-NeoLib: A falsifiable targeted LC-MS/MS discovery hypothesis for menstrual transgelin neo-fragments in adenomyosis

Key Points

• This research aims to determine if specific transgelin-derived neo-fragments are detectable in menstrual blood from patients with adenomyosis.
• Utilized a targeted LC-MS/MS approach to analyze menstrual blood samples.
• Included clinically relevant comparator groups to assess neo-fragment presence.
• Established strict Go/No-Go rules before potential immunoassay development.
• Proposed neo-fragments are expected to be reproducibly enriched in menstrual blood from adenomyosis patients.
• Validation as a diagnostic biomarker is not claimed at this stage.
• The approach emphasizes the necessity of robustness in result reproducibility before further development.

Abstract