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May 9, 2026JBMR Plus0 citationsOpen Access

Brain and Knee Joint Degeneration Following Anterior Cruciate Ligament Injury in a Mouse Model of Alzheimer’s Disease

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WYWei YaoBWBarton L. WiseYLYu‐Yang Lin

Key Points

  • The study aims to explore the relationship between knee joint degeneration and cognitive decline in a mouse model of Alzheimer's Disease.
  • Investigated brain and knee joint degeneration in APP/PS1 double transgenic and wild-type mice after non-invasive ACL rupture.
  • Conducted neurocognitive tests including open field, novel object recognition, and Morris water maze 6 weeks post-injury.
  • Quantified osteophyte formation and analyzed beta amyloid and phosphorylated tau levels in brain and knee joints at 6 weeks post-injury.
  • Female APP/PS1-OA mice exhibited significantly more osteophyte formation than WT-OA mice.
  • Increased levels of beta amyloid and phosphorylated tau were found in the brain and knee joints of APP/PS1-OA mice compared to WT or APP/PS1-Sham mice.
  • Findings suggest a connection between OA-related inflammation and cognitive dysfunction.

Abstract

Abstract Alzheimer’s Disease (AD) and osteoarthritis (OA) are two of the most common health conditions affecting the elderly. Recent studies in mice and humans have described an association between OA and AD, with prevalent OA increasing the risk of developing AD-like cognitive declines. In this study, we investigated brain and knee joint degeneration and neurocognitive dysfunction in a mouse model of AD (APP/PS1 double transgenic mice) in the presence or absence of OA induced by non-invasive ACL rupture. We hypothesized that OA would be associated with increased beta amyloid (Aβ) and phosphorylated tau (pTau) in the injured joint and brain and would be associated with accelerated cognitive decline in APP/PS1-Tg mice. Non-invasive knee injury was performed at 7 months of age in male and female APP/PS1-Tg mice and non-transgenic (WT) littermates. Neurocognitive tests including open field, novel object recognition, and Morris water maze were performed 6 weeks post-injury. Knees were also assessed at 6 weeks post-injury to quantify OA, and Aβ, Tau, and pTau levels in the brain and knee joints. We found that female APP/PS1-OA mice had significantly more osteophyte formation than WT-OA mice, and significantly greater Aβ and pTau levels in the brain and knee joints than in WT or APP/PS1-Sham mice. These results provide insights about the pathoetiology of AD and OA and suggest possible common mechanisms connecting OA-associated inflammation to cognitive function. Identifying novel mechanisms of crosstalk between these two conditions could improve clinical care and quality of life for at-risk patient populations.

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Cite This Study

Yao et al. (2026) studied this question.

synapsesocial.com/papers/69fecfafb9154b0b82876ab6https://doi.org/10.1093/jbmrpl/ziag085
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