Total Chemical Synthesis of the Human Milk Oligosaccharide Disialyllacto- N -tetraose and Its Degradation by Bifidobacterium bifidum Sialidases

Key Points

• The research aims to achieve total chemical synthesis of disialyllacto-n-tetraose and investigate its degradation by specific sialidases.
• Conducted total chemical synthesis of disialyllacto-n-tetraose.
• Analyzed the degradation process by isolating sialidases from bifidobacterium bifidum.
• Successfully synthesized disialyllacto-n-tetraose using a total chemical approach.
• Observed significant degradation of synthesized oligosaccharide by bifidobacterium bifidum sialidases.