Screening, Characterization, and Engineering of Universal Promoters for Bifidobacterium breve

Key Points

• The aim is to identify and engineer universal promoters that improve gene expression in Bifidobacterium breve.
• Screening of various promoter sequences in Bifidobacterium breve
• Characterization of promoter efficiency through gene expression assays
• Engineering of selected promoters to optimize functions
• Identification of multiple universal promoters with varying expression levels
• Successful enhancement of gene expression in engineered strains
• Promoters demonstrate compatibility with multiple Bifidobacterium species