Objectives/Goals: Quantify environmental persistence and airborne stability of avian (aMPV) and human metapneumovirus (hMPV) and evaluate far-UVC light (at 222 nm) as a safe, non-chemical disinfection method to reduce viral transmission in agricultural and healthcare environments. Methods/Study Population: Subtypes A and B of aMPV and subtype B of hMPV isolates will be propagated in Vero and LLC-MK2 cells, respectively. Viral persistence will be quantified on fomites under varying temperature and humidity. Aerosol stability will be assessed using an environmental chamber and an atomizer system. Once baseline survival is established, samples and aerosols will be exposed to different far-UVC doses, intensities, and durations. The endpoints will be determined by observing cytopathic effects (CPE), virus titers will be calculated by the Karber method, and titers will be expressed as TCID50. Results/Anticipated Results: We anticipate quantifying surface and aerosol stability profiles of aMPV and hMPV across several porous and non-porous materials (fomites) and environmental conditions relevant to poultry and clinical settings. Far-UVC exposure is predicted to achieve rapid viral inactivation on non-porous surfaces and in aerosols without measurable cytotoxicity or material degradation. The findings will establish disinfection thresholds and demonstrate the practicality of far-UVC integration into real-world agricultural and healthcare biosecurity systems. Discussion/Significance of Impact: This work advances One Health biosecurity by establishing safe far-UVC disinfection protocols to control the spread of both aMPV and hMPV. Findings will inform infection control policies and reduce economic and health burdens in poultry operations and vulnerable human populations.
Hatfield et al. (Wed,) studied this question.
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