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May 9, 2026Journal of Agricultural and Food Chemistry0 citations

Structure-Guided Engineering of UGT94B1 M0 Enhances Thermostability and Enables Efficient Rebaudioside D Biosynthesis via Coupled UDP-Glucose Regeneration

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ZLZhengshan LuoXGXupeng GuoZDZhiwei Deng

Key Points

  • This research aims to improve the thermostability of UGT94B1 for better glycoside biosynthesis.
  • Engineered UGT94B1 M0 for enhanced thermostability via structural guidelines.
  • Focused on the regeneration of UDP-glucose to boost glycosylation efficiency.
  • Demonstrated significant improvement in thermostability of UGT94B1 M0.
  • Achieved effective biosynthesis of rebaudioside D using engineered enzyme.

Abstract

SuSy. This work establishes a generalizable strategy for thermostability engineering of UDP-glycosyltransferases toward efficient and sustainable glycoside biosynthesis.

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Cite This Study

Luo et al. (2026) studied this question.

synapsesocial.com/papers/69fed0f8b9154b0b82878221https://doi.org/10.1021/acs.jafc.5c17374
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