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May 9, 2026Tropical Journal of Natural Product Research0 citationsOpen Access

Bao Thanh Syrup Attenuates Carrageenan-Induced Pulmonary Inflammation in Rats: Evidence for Dose-Dependent Anti-inflammatory and Cytokine-Modulatory Activity

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TTTung T. TranPPPhong X. PhamLNLoan TT. Nguyen

Key Points

  • To assess the anti-inflammatory effects of Bao Thanh syrup in a carrageenan-induced pulmonary inflammation model in rats.
  • Rats were assigned to five groups (n = 24 each) and treated with vehicle, dexamethasone, or Bao Thanh syrup at two dosages (5.4 or 16.2 ml/kg/day).
  • Pulmonary inflammation was induced using carrageenan (2 mg/kg) via intratracheal administration, and various inflammatory markers were measured after 24 hours.
  • Parameters assessed include lung edema, inflammatory cell counts in bronchoalveolar lavage fluid and blood, cytokine levels, oxidative stress markers, and histopathological changes.
  • BT at 16.2 ml/kg/day significantly reduced lung edema, inflammatory cell infiltration, and TNF-α levels in BALF and blood.
  • Elevated levels of WBC, LYMP, and NEU were significantly attenuated in the treatment group receiving the higher dosage of BT.
  • BT at 5.4 ml/kg/day showed a non-significant trend toward anti-inflammatory activity in the model.

Abstract

Pneumonia reduces quality of life and imposes economic burdens, underscoring the need for alternative treatments. Our study aimed to evaluate the potential anti-inflammatory effects of Bao Thanh syrup (BT) in a carrageenan-induced pulmonary inflammation model in rats. Pulmonary inflammation was induced via intratracheal administration of carrageenan (2 mg/kg) in adult Wistar rats randomly assigned to five groups (n = 24 per group). Animals were treated with either vehicle, dexamethasone (2 mg/kg/day), or BT (5.4 or 16.2 ml/kg/day). After 24 hours, lung edema (relative lung weight, wet/dry ratio), inflammatory cell counts (total leukocyte (WBC), lymphocyte (LYMP), and neutrophil (NEU)) in bronchoalveolar lavage fluid (BALF) and blood, cytokine levels, C-reactive protein (CRP), oxidative stress markers, and histopathological changes were assessed. Intratracheal instillation of carrageenan induced significant pulmonary inflammation, evidenced by increased lung oedema, elevated WBC, LYMP, and NEU counts in both BALF and blood, and elevated CRP levels. BT at 16.2 ml/kg/day significantly attenuated these changes, reducing inflammatory cell infiltration, TNF-α levels in BALF and blood, and histopathological lung injury. Although IL-6 and IL-1β levels showed a decreasing trend, the changes were not statistically significant. BT at 5.4 ml/kg/day exhibited a trend toward anti-inflammatory activity in the carrageenan-induced pulmonary inflammation model in rats. Bao Thanh syrup demonstrated dose-dependent anti-inflammatory activity in a carrageenan-induced pulmonary inflammation model in rats, suggesting its potential as an adjunctive agent for inflammatory lung conditions and providing a preclinical basis for further investigation in infectious pneumonia models.

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Cite This Study

Tran et al. (2026) studied this question.

synapsesocial.com/papers/69fed17eb9154b0b82878d85https://doi.org/10.26538/tjnpr/v10i4.16
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