Offline Two-Stage SEC and LC-MS/MS for Comprehensive Characterization of Yeast Ribosomal Populations

Puntos clave

• This research aims to characterize yeast ribosomal populations and investigate their functional changes during polysome collapse.
• Utilized offline two-stage size exclusion chromatography (SEC) combined with LC-MS/MS for analysis.
• Focused on detecting changes in ribosome composition during functional perturbations.
• Employs polysome profiling to assess the interactions and compositions of ribosomal populations.
• Demonstrated rapid polysome collapse in yeast under knockout conditions.
• Detected significant alterations in polysome profiling ratios linked to ribosomal composition changes.