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May 11, 2026BMC Women s Health0 citationsOpen Access

Effects of short-term resistance training plus whole-body neuromuscular electrical stimulation on blood cardiometabolic biomarkers in sedentary middle-aged women: a parallel-group, randomized trial

PAPaulo Vitor Suto AizavaVPVitor de Salles PainelliLSLeonardo de Paula Silva

Key Result

Six weeks of resistance training combined with whole-body neuromuscular electrical stimulation accelerated reductions in LDL-c and total cholesterol, but lacked statistical superiority over RT alone.

Key Points

  • The study aims to evaluate whether short-term resistance training with neuromuscular electrical stimulation can improve blood cardiometabolic biomarkers in sedentary middle-aged women.
  • Participants (N=23) were randomized to six weeks of resistance training with neuromuscular electrical stimulation or resistance training alone, engaging in two sessions per week.
  • Primary outcomes included blood biomarkers like total cholesterol and LDL-c, while secondary outcomes monitored anthropometric measures and physical fitness.
  • No significant interactions were found for any blood biomarker, with significant time effects noted for LDL-c (P=0.002) and total cholesterol (P=0.001).
  • The RT + WB-NMES group saw reductions in LDL-c (P=0.041, ES=-1.13) and total cholesterol (P=0.026, ES=-0.91).
  • Lumbar traction strength improved significantly in the RT + WB-NMES group (P=0.029, ES=0.99), with no change in the RT group (P=0.569, ES=0.53).

Study Design

Type

RCT (n=23)

Blinding

Single-blind

Randomization

Simple randomization sequence

Multicenter

No

Structured PICO

Does resistance training combined with whole-body neuromuscular electrical stimulation improve blood cardiometabolic biomarkers in sedentary middle-aged women?

P
Population
23 sedentary middle-aged women (aged 35-55 years), mean age 47. Key inclusion: <150 min/week of moderate-to-vigorous physical activity over the previous 6 months, no structured exercise in 6 months. Exclusion: cardiovascular, metabolic, or musculoskeletal conditions.
I
Intervention
Resistance training (RT) combined with whole-body neuromuscular electrical stimulation (WB-NMES) for 6 weeks, performing two supervised 30-minute sessions per week.
C
Comparator
Resistance training (RT) alone for 6 weeks, performing two supervised 30-minute sessions per week.
O
Outcome
Blood biomarkers related to cardiometabolic risk (total cholesterol, LDL-c, HDL-c, VLDL-c, triglycerides, fasting glucose, liver enzymes, and C-reactive protein) at 6 weeks.surrogate

Six weeks of resistance training combined with whole-body neuromuscular electrical stimulation accelerated reductions in LDL-c and total cholesterol in sedentary middle-aged women, though it was not statistically superior to resistance training alone.

Limitations

  • Small sample size
  • Short intervention duration (6 weeks)
  • Lack of participant and instructor blinding

Abstract

BACKGROUND: Middle-aged women are disproportionately affected by cardiometabolic deterioration during the menopausal transition. Resistance training (RT) is an established countermeasure, and whole-body neuromuscular electrical stimulation (WB-NMES) may enhance its physiological effects. However, whether short-term RT combined with WB-NMES further improves blood cardiometabolic biomarkers in this population remains unknown. METHODS: ) were allocated to six weeks of RT combined with WB-NMES (RT + WB-NMES; n = 11) or RT alone (RT; n = 12), performing two supervised sessions per week. Primary outcomes were blood biomarkers related to cardiometabolic risk (total cholesterol, LDL-c, HDL-c, VLDL-c, triglycerides, fasting glucose, liver enzymes, and C-reactive protein). Secondary outcomes comprised anthropometric measures, body composition, and physical fitness. RESULTS: No significant Group × Time interaction was identified for any blood biomarker. A significant main effect of Time was observed for LDL-c (P = 0.002) and total cholesterol (P = 0.001), with post-hoc analyses revealing significant within-group reductions exclusively in the RT + WB-NMES group (LDL-c: P = 0.041, ES = - 1.13; total cholesterol: P = 0.026, ES = - 0.91). No significant Group × Time interaction was found for any anthropometric, body composition, or most physical fitness variables. Lumbar traction strength improved significantly within the RT + WB-NMES group (P = 0.029, ES = 0.99) but not in RT alone (P = 0.569, ES = 0.53). CONCLUSIONS: Six weeks of RT combined with WB-NMES accelerated reductions in LDL-c and total cholesterol in sedentary middle-aged women, although statistical superiority over RT alone was not demonstrated. These preliminary findings support the cardiometabolic relevance of short-term RT + WB-NMES, though larger trials are required. TRIAL REGISTRATION: U111113186998 https://ensaiosclinicos.gov.br/rg/RBR6zx5zcz. Registered on 10/09/2025.

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Cite This Study

Aizava et al. (2026) conducted an RCT in Sedentary middle-aged women (n=23). Resistance training plus whole-body neuromuscular electrical stimulation (RT+WB-NMES) vs. Resistance training (RT) alone was evaluated on Blood cardiometabolic biomarkers (Group × Time interaction). Six weeks of resistance training combined with whole-body neuromuscular electrical stimulation accelerated reductions in LDL-c and total cholesterol, but lacked statistical superiority over RT alone.

synapsesocial.com/papers/6a0171983a9f334c28271afchttps://doi.org/10.1186/s12905-026-04465-4
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