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May 13, 2026MoleculesOpen Access

A Poultry Universal Primer-Based Fluorescent PCR (PUP-fPCR) for Simultaneous Identification and Quantification of Chicken, Quail, Duck, and Goose Meat Species

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Authors

YLYifan LiHCHaoyang CaoGCGuangxiang Chen

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Overview

Randomized trial develops a PCR method to identify chicken, quail, duck, and goose meat, suggesting a new tool against food fraud.

Key Points

  • The aim is to develop a method that identifies and quantifies multiple poultry species in meat products to prevent adulteration.
  • Developed a multiplex quantitative PCR method using universal primers and TaqMan probes.
  • Evaluated analytical performance using prepared DNA mixtures for accuracy and precision.
  • Targeted conserved nuclear DNA sequences unique to different poultry species.
  • Achieved a limit of detection at 0.005 ng with high specificity and sensitivity.
  • Demonstrated robust accuracy with a relative deviation of 0.80–5.05%.
  • Maintained precision with a relative standard deviation of 0.94–13.84%.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/6a0414f679e20c90b4444d75https://doi.org/10.3390/molecules31101590
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