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May 14, 2026INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY0 citationsOpen Access

Galbibacter kalidii sp. nov., a flexirubin-type pigment-producing bacterium, isolated from saline soil in Xinjiang

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YHY HuangYYYi YuanJMJinbiao Ma

Key Points

  • The aim is to characterize a novel bacterial species, Galbibacter kalidii sp. nov., isolated from saline soil.
  • Isolated strain EGI 63066 T from saline soil in Xinjiang, China.
  • Conducted 16S rRNA sequencing and phylogenomic analysis to assess genetic relationships.
  • Analyzed fatty acid profiles, major respiratory quinones, and polar lipids for taxonomy.
  • Strain EGI 63066 T shares 92.80% 16S rRNA similarity with Galbibacter mesophilus.
  • Average nucleotide identity values with Galbibacter members range from 71.10% to 74.15%.
  • EGI 63066 T produces flexirubin-type pigments and shows unique whole-cell protein profiles.

Abstract

A Gram-stain-negative, strictly aerobic, rod-shaped bacterium, designated EGI 63066 T , was isolated from a saline soil of Kalidium foliatum (Pall.), collected from Wujiaqu in Xinjiang, China. Strain EGI 63066 T is a non-motile, rod-shaped bacterium; it forms pale yellow colonies on the marine agar and grows at 10–37 °C,at pH 6.0–9.0 and 0–10% (w/v) NaCl. The 16S rRNA sequences of the strain EGI 63066 T and Galbibacter mesophilus Mok-17 T , Robertkochia solimangrovi CL23 T , Robertkochia sediminum 1368 T and Galbibacter pacificus CMA-7 T share sequence similarities of 92.80%, 91.85%, 91.79% and 91.79%, respectively. Higher average amino acid identity values with Galbibacter (72.10–76.90%) than with Robertkochia (69.40–70.30%) supported the assignment of strain EGI 63066 T to the genus Galbibacter . Phylogenomic analysis based on core genes revealed that strain Galbibacter kalidii formed a stable clade with the four recognized species of the genus Galbibacter and the not validly published ‘ Joostella atrarenae ’ M1-2. The average nucleotide identity and digital DNA–DNA hybridization values between the strain EGI 63066 T and members of the genus Galbibacter were 71.10–74.15% and 18.00–19.70%, respectively. The DNA G+C content of the genome for strain EGI 63066 T based on genomic DNA was 39.0 mol%, and the genome size was 4.66 Mbp. The predominant fatty acids of strain EGI 63066 T were iso-C 15 : 0 , iso-C 15 : 1 G and summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c). The major respiratory quinone was menaquinone-6, and the major polar lipids were phosphatidylethanolamine, aminolipid, aminophospholipid and phospholipid. Compared to other species within the genus Galbibacter , EGI 63066 T exhibits distinctive features, including the production of flexirubin-type pigments, CM-cellulose hydrolysis and whole-cell protein profiles. Based on the polyphasic taxonomic analysis, strain EGI 63066 T represents a novel species of the genus Galbibacter , for which the name G. kalidii sp. nov. is proposed. The type strain is EGI 63066 T (=JCM 36938 T =CGMCC 1.19132 T ).

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Cite This Study

Huang et al. (2026) studied this question.

synapsesocial.com/papers/6a0567a8a550a87e60a1fd84https://doi.org/10.1099/ijsem.0.007132
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