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May 14, 2026Physiology

Assessment of Exercise-Like Electrical Pulse Stimulation on SIRT1 Signaling and Mitochondrial Function in Ethanol-Treated Myotubes

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Authors

KRKeishla Rodríguez-GracianiZDZaidmara DiazLSLiz Simon

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Overview

Randomized trial investigates EPS effects on mitochondrial function in ethanol-treated myotubes, suggesting recovery of metabolic health.

Key Points

  • This study aims to assess if electrical pulse stimulation can reverse mitochondrial dysfunction due to ethanol exposure in myotubes.
  • C2C12 myoblasts were differentiated into myotubes and exposed to ethanol and/or EPS.
  • SIRT1/SIRT3 protein expression and PGC-1α acetylation were analyzed by Western blot.
  • Mitochondrial function was measured using Seahorse XF Pro Mito Stress Test and live-cell imaging.
  • Ethanol decreased SIRT1 (p<0.001) and SIRT3 (p<0.05) and increased PGC-1α acetylation.
  • Ethanol impaired mitochondrial respiration, reducing spare respiratory capacity (p<0.01).
  • EPS improved mitochondrial function, restoring oxygen consumption rates and spare respiratory capacity (p<0.05) in ethanol-treated cells.

Cite This Study

Rodríguez-Graciani et al. (2026) studied this question.

synapsesocial.com/papers/6a0567d2a550a87e60a2013chttps://doi.org/10.1152/physiol.2026.41.s1.2301230
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