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May 14, 2026Physiology0 citations

Brachial artery blood flow kinetics during low-intensity dynamic handgrip exercise in patients with a Fontan circulation

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KHKayla B. HarderAGAustin J. GallantMKM. Rafique Khan

Key Result

Low-intensity dynamic handgrip exercise produced comparable blood flow kinetics in Fontan patients and healthy controls (tau 22 vs 23 s, P=0.702), despite lower steady-state blood flow.

Key Points

  • This study aims to determine how peripheral blood flow kinetics are affected during low-intensity handgrip exercise in patients with Fontan circulation.
  • Participants included patients with Fontan circulation (FTN) and age- and sex-matched healthy controls (CTL).
  • Each group completed three bouts of dynamic handgrip exercise for 5 minutes at 20% maximal voluntary contraction.
  • Brachial artery diameter and velocity were recorded using Doppler ultrasound and analyzed to compute blood flow.
  • FTN group exhibited a lower amplitude increase in blood flow (14 ± 7 mL/min) compared to CTL (25 ± 16 mL/min, P=0.014).
  • Steady-state exercise blood flow was also lower in FTN (38 ± 11 mL/min) versus CTL (53 ± 25 mL/min, P=0.031).
  • Blood flow kinetics, including time delay and tau, were comparable between FTN and CTL.

Study Design

Type

Cross-Sectional (n=31)

Structured PICO

Does low-intensity dynamic handgrip exercise alter peripheral blood flow kinetics in patients with a Fontan circulation compared to healthy controls?

P
Population
31 participants, including 18 patients with Fontan circulation (FTN; 9 male/9 female, age 16 ± 4 years) and 13 age- and sex-matched healthy controls (CTL; 7 male/6 female, age 17 ± 4 years).
I
Intervention
Three bouts of dynamic handgrip exercise for 5 min at 20% of maximal voluntary contraction.
C
Comparator
Healthy controls undergoing the same dynamic handgrip exercise protocol.
O
Outcome
Peripheral blood flow kinetics (time delay, time constant tau, mean response time) and amplitude increase in blood flow measured by Doppler ultrasound.surrogate

In patients with a Fontan circulation, peripheral blood flow kinetics during low-intensity exercise are comparable to healthy controls, but the amplitude of blood flow increase is lower, indicating differences in vascular control.

Main Result

Absolute Event Rate: 22% vs 23%

p-value: p=0.702

Abstract

Fontan circulation (FTN) is associated with exercise intolerance, but the contribution of peripheral blood flow to this limitation is unclear. This study aimed to determine whether peripheral blood flow kinetics are altered in response to low-intensity dynamic handgrip exercise. It was hypothesized that patients with FTN would exhibit slowed blood flow kinetics. To test this hypothesis, FTN (9M/9F, 16 ± 4 years of age) and similar age- and sex-matched healthy controls (CTL, 7M/6F, 17 ± 4 years of age, P=0.614) completed three bouts of dynamic handgrip exercise for 5 min at 20% of maximal voluntary contraction (FTN: 10 ± 4 kg vs CTL: 18 ± 7 kg, P< 0.001). Brachial artery diameter and blood flow velocity were continuously recorded using Doppler ultrasound (Vivid i, GE Medical Systems), analyzed offline (Cardiovascular Suite 4.3.0, Quipu, Italy), and used to calculate blood flow. Second-by-second blood flow from each bout were time-aligned and averaged to obtain a single response profile for each participant. Subsequently, blood flow data was further averaged into 5-s bins and the first 3 min of the exercise were modelled using monoexponential curve fitting (OriginLab, OriginPro 2024b). FTN and CTL had similar baseline blood flow (FTN: 24 ± 11 mL/min vs CTL: 27 ± 14 mL/min, P=0.553). The amplitude increase in blood flow was lower in FTN (14 ± 7 mL/min) vs CTL (25 ± 16 mL/min, P=0.014), owing to the lower absolute exercise intensity. Accordingly, steady-state exercise blood flow was also lower in FTN (38 ± 11 mL/min) vs CTL (53 ± 25 mL/min, P=0.031). The time delay for the increase in blood flow was comparable between FTN (0.03 ± 0.36 s) and CTL (-0.05 ± 0.26 s, P=0.507). The blood flow time constant, tau, was also comparable between FTN (22 ± 8 s) and CTL (23 ± 11 s, P=0.702), as was the mean response time (time delay + tau, FTN: 22 ± 8 s vs CTL: 24 ± 10 s, P=0.560). During the exercise transient, FTN showed a 7% smaller reduction in vessel diameter and a 33% lower increase in blood velocity than CTL. These data indicate that the kinetics of blood flow are comparable between FTN and CTL, but that the control of blood flow differs. Funding: Saskatchewan Centre for Patient Oriented Research, Canadian Institute of Health Research, Scottish Rite Charitable Foundation of Canada, Heart and Stroke Foundation of Canada) This abstract was presented at the American Physiology Summit 2026 and is only available in HTML format. There is no downloadable file or PDF version. The Physiology editorial board was not involved in the peer review process.

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Cite This Study

Harder et al. (2026) conducted a cross-sectional in Fontan circulation (n=31). Low-intensity dynamic handgrip exercise vs. Healthy controls was evaluated on Blood flow time constant (tau) (p=0.702). Low-intensity dynamic handgrip exercise produced comparable blood flow kinetics in Fontan patients and healthy controls (tau 22 vs 23 s, P=0.702), despite lower steady-state blood flow.

synapsesocial.com/papers/6a05680ea550a87e60a2069chttps://doi.org/10.1152/physiol.2026.41.s1.2345165
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