OGG1 deletion inhibites the glycolytic pathway of cardiomyocytes

Diabetes is the most common metabolic disease worldwide, and Diabetic Cardiomyopathy (DCM) is a severe complication of diabetes. Energy metabolism disruption is a key factor in the development of DCM and subsequent heart failure. It is well known that changes in glucose metabolism can lead to severe disruptions in lipid, amino acid, and energy metabolism, causing lasting damage to various tissues and organs. To better understand the impact of OGG1 on the metabolic changes caused by DCM, the study investigated whether OGG1 affects glucose metabolism in cardiac muscle cells, conducting a comprehensive targeted metabolomics analysis on PA-induced H9C2 cells. Initially, the OGG1 gene in H9C2 cells was silenced using RNA interference techniques, followed by stimulating the cells with solvent and PA separately for 24 hours, collecting all cell precipitates for metabolomics analysis.