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May 15, 2026Frontiers in Genetics0 citationsOpen Access

Extracellular vesicle–associated and non–vesicle-associated microRNAs for early breast cancer detection: a systematic review

JUJessica Daniela Romero UrregoCPCarlos Alberto Parra-LópezMIMario Arturo Isaza-Ruget

Key Points

  • This review aims to evaluate the diagnostic performance of circulating microRNAs for early detection of breast cancer. It focuses on both exosomal and non-exosomal miRNAs as potential biomarkers.
  • Systematic review of studies published between 2016 and 2025.
  • Evaluated diagnostic accuracy of individual and panel microRNAs derived from blood samples.
  • Examined performance metrics such as Area Under the Curve (AUC) in differentiating breast cancer patients from controls.
  • Individual miRNAs, including miR-21-5p and miR-200c, showed high diagnostic accuracy with AUC > 0.9.
  • Panels comprising miR-106a-3p, miR-106a-5p, miR-20b-5p, and miR-92a-2-5p demonstrated similar high levels of accuracy.
  • Challenges noted include tumor heterogeneity and issues with standardization of pre-analytical processes.

Abstract

Breast cancer remains a leading cause of morbidity and mortality worldwide, with survival outcomes strongly dependent on early detection. Conventional mammography, while widely adopted, faces limitations in sensitivity—particularly in dense breast tissue—and is not without invasiveness. Circulating microRNAs (miRNAs), both exosomal and non-exosomal, have emerged as promising non-invasive biomarkers due to their stability in biofluids and cancer-associated dysregulation. This systematic review evaluated studies published between 2016 and 2025 assessing the diagnostic performance of blood-derived miRNAs for early breast cancer detection. Several individual miRNAs, including miR-21-5p and miR-200c, as well as panels such as those comprising miR-106a-3p, miR-106a-5p, miR-20b-5p, and miR-92a-2-5p, demonstrated high diagnostic accuracy (Area Under the Curve (AUC) 0.9) in distinguishing breast cancer patients from healthy controls. Despite challenges related to tumor heterogeneity and pre-analytical standardization, integrating circulating miRNA biomarkers with current imaging modalities holds significant potential to enhance early detection and support personalized management strategies.

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Cite This Study

Urrego et al. (2026) studied this question.

synapsesocial.com/papers/6a06b74ce7dec685947aa42ahttps://doi.org/10.3389/fgene.2026.1716692
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