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May 15, 2026Journal of Agricultural and Food Chemistry0 citations

Computational-Aided Mutagenesis of Lipase CRL1 with Promoted Stability and Activity for Biosynthesis of Vitamin E Succinate

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WSWenyu ShaoYZYu ZhangJWJialing Wang

Key Points

  • The aim is to enhance the stability and activity of lipase CRL1 through computational-aided mutagenesis to support vitamin E succinate biosynthesis.
  • Generated mutant lipase variants using computational techniques.
  • Evaluated the yield of vitamin E succinate at optimized conditions (60 °C for 12h).
  • Mutant 6M achieved a 6.8-fold increase in yield compared to wild type.
  • Yield for mutant 6M reached 77.8% under optimal conditions.

Abstract

) showed a 6.8-fold improvement over that of WT. Mutant 6M could generate vitamin E succinate with an improved yield of 77.8% (60 °C, 12 h). The synergistically functional modifications of the lipase pave the way for the mutated lipase to become a highly promising and robust biocatalyst for industrial applications.

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Cite This Study

Shao et al. (2026) studied this question.

synapsesocial.com/papers/6a06b7a1e7dec685947aa65chttps://doi.org/10.1021/acs.jafc.6c02908
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