In vitro and in silico studies reveal anti-obesity effects of metabolites in 3T3-L1 cells, suggesting new treatment avenues.
Key Points
This research aims to investigate the anti-obesity effects of secondary metabolites from Chrysosplenium flagelliferum by examining their impact on lipid accumulation.
In vitro experiments using 3T3-L1 cells to assess lipid accumulation inhibition by cucurbitacin D and cirsimaritin.
Comparison of binding mechanisms to PPAR-γ between the two metabolites.
Cucurbitacin D inhibits lipid accumulation by downregulating PPAR-γ through flexible binding.
Cirsimaritin also inhibits lipid accumulation but employs stable, compact docking, indicating different mechanisms.