Covalent Chemical Tagging of Transmembrane Transport Proteins Illuminates the Internalization Pathways of Xenosiderophores

Key Points

• The study aims to investigate the role of the cobalamine transporter BtuB in the transport of DFO derivatives.
• Utilized covalent photo-cross-linking for structural design of siderophore conjugates
• Analyzed the transport mechanisms involving BtuB and xenosiderophores
• Explored the internalization pathways of metal ions in bacteria
• Identified a new role for BtuB in the transport of DFO derivatives
• Demonstrated effective covalent tagging of transmembrane proteins using siderophore conjugates
• Provided insights into the mechanisms of metal ion uptake across bacterial membranes

Abstract