β-Hydroxy Thioether-Derived Ionizable Lipids for Spleen-Tropic mRNA Delivery and In Vivo Chimeric Antigen Receptor T Cell Engineering

Puntos clave

• To explore the use of ionizable lipids for mRNA delivery to engineer CAR T cells targeting spleen tissues.
• Utilized β-hydroxy thioether-derived ionizable lipids for the formulation of lipid nanoparticles.
• Evaluated delivery efficiency of mRNA in vivo for chimeric antigen receptor T cell production.
• Effective delivery of mRNA via lipid nanoparticles to generate functional CAR T cells.
• Targeting of T cells enhanced in spleen tissues compared to other methods.