The main aim is to develop an efficient method for the automated immobilization of dual enzymes.
Developed a directed automated immobilization platform for enzymes.
Utilized SpyTag/SpyCatcher chemistry for enzyme coupling.
Analyzed the efficiency of enzyme activity post-immobilization.
Showed high efficiency in enzyme immobilization rates.
Demonstrated enhanced performance of dual enzymes compared to traditional methods.
Abstract
@β-Gal + LFAI). The sequential and directed automated immobilization platform developed by our research provides a novel approach for the efficient immobilization of dual enzymes.