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May 17, 2026International Journal of Infectious Diseases1 citationsOpen Access

Effectiveness of an Information-Driven Targeted Isolation Strategy in Reducing Healthcare-Associated Infections Caused by Multidrug-Resistant Gram-Negative Bacteria in the Intensive Care Unit: A Prospective Uncontrolled Before-After Study

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PTPing TianAMAdila MaimaitiYZYuan Zhou

Key Points

  • This study aims to evaluate the effectiveness of a targeted isolation strategy based on a risk calculator to reduce MDR-GNB infections in ICUs.
  • Conducted a prospective uncontrolled before-after study across four adult ICUs in two hospitals.
  • Implemented a 2-week washout period between standard precautions and targeted isolation phases.
  • Used a risk calculator for identifying high-risk patients for isolation during the intervention phase.
  • The MDR-GNB HAI rate during intervention was 0.46% (2 out of 435) compared to 1.98% (13 out of 656) during control (p = 0.035).
  • The intervention was feasible and significantly reduced the transmission of MDR-GNB infections.

Abstract

BACKGROUND: Cross-transmission risk of multidrug-resistant Gram-negative bacteria (MDR-GNB) is substantially higher in intensive care units (ICUs). Broad patient isolation is resource-intensive, prompting this study to assess whether a validated MDR-GNB healthcare-associated infection (HAI) risk calculator-guided targeted preemptive isolation could reduce cross-transmission. METHODS: A prospective uncontrolled before-after study was conducted across four adult ICUs in two tertiary hospitals in Urumqi, with two 3-month phases separated by a 2-week washout period. Standard precautions were applied in the control phase, while the intervention phase used the risk calculator for targeted preemptive isolation of high-risk patients. The primary outcome was ICU-acquired MDR-GNB infection (ICUAI) incidence. RESULTS: Among a total of 2,101 ICU admissions, 1,091 patients met the inclusion criteria and were included in the analysis. The rate of MDR-GNB healthcare-associated infection (HAI) was significantly lower during the intervention periods (2 out of 435, 0.46%) than during the control periods (13 out of 656, 1.98%; p = 0.035). CONCLUSION: Despite the relatively short duration of this trial, the use of an MDR-GNB HAI risk calculator to guide the preemptive isolation of high-risk patients was both feasible and effective in reducing transmission. TRIAL REGISTRATION NO: ChiCTR2500113287, retrospectively registered.

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Cite This Study

Tian et al. (2026) studied this question.

synapsesocial.com/papers/6a095ac47880e6d24efe091bhttps://doi.org/10.1016/j.ijid.2026.108790
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