Objectives This study aims to investigate the spatial transcriptomics of hypoxia and immune‐mediated damage within renal tissue in patients with lupus nephritis (LN) in comparison to healthy controls from kidney transplants. Methods Renal biopsies from LN patients and transplant controls were imaged using multiplexed cyclic immunofluorescence to spatially quantify immune and endothelial cell markers. An independent LN cohort of patients underwent Visium spatial transcriptomics analysis. Hypoxia was modeled in cell lines and primary neutrophils using cobalt chloride, while urinary neutrophil cargo proteins were measured using ELISA . Results LN glomeruli exhibited increased HIF1α expressing CD68 +ve macrophages and CD66 +ve neutrophils along with diffuse perinuclear staining for myeloperoxidase (MPO), indicative of heightened NETosis and N2 reparative neutrophil presence. Spatial transcriptomic analysis confirmed the correlation between glomerular hypoxia and NET formation. The renal pathology Activity Index (AI) was highly correlated with hypoxic neutrophil count, and these parameters correlated with glomerular endothelial rarefaction, and reduced capillary lengths, junctions, and branching. Urinary neutrophil cargo and NET proteins (MPO and proteinase 3) were also elevated in active LN. Conclusions We posit that glomerular endothelial cell damage leads to hypoxia in LN, resulting in hypoxic, reparative myeloid cells and neutrophil NETosis, while the latter has been linked to downstream inflammation and in situ trapping of anti‐nuclear antibodies in LN. These glomerular events underpin high renal pathology activity, and may pave the path towards irreversible damage in LN. Detection of neutrophil cargo in urine supports the potential for a liquid biopsy to monitor disease progression
Titus et al. (2026) studied this question.