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May 17, 2026ChemElectroChem0 citationsOpen Access

Optimizing Microbial Fuel Cell Efficiency Through a Designed Electrogenic Consortium to Remove Alkylbenzenes

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JSJoão Carlos de SouzaAZAna Clara Bonizol ZaniVRValéria Reginatto

Key Points

  • This research aims to enhance the biodegradation of alkylbenzenes using a novel electrogenic microbial consortium in microbial fuel cells.
  • Developed a microbial fuel cell with a novel electrogenic consortium from mangrove sediments.
  • Progressively acclimated the consortium to efficiently biodegrade ethylbenzene and xylenes while producing electricity.
  • Conducted electrochemical and microscopic characterizations to analyze performance and biofilm properties.
  • Achieved a maximum voltage of 520.0 ± 15.7 mV and a power density of 63.4 ± 5.3 mW m−2.
  • Removed 97.2 ± 1.9% of xylenes and 89.4 ± 7.5% of ethylbenzene over 120 hours.
  • Demonstrated enhanced electrochemical performance through improved extracellular electron transfer and increased biofilm conductivity.

Abstract

Developing sustainable remediation strategies to biodegrade alkylbenzenes remains challenging. We have developed a microbial fuel cell (MFC) based on a novel electrogenic microbial consortium derived from mangrove sediments. The consortium was progressively acclimated to codegrade ethylbenzene and xylenes (EXs) while generating bioelectricity, achieving a maximum voltage of 520.0 ± 15.7 mV and a power density of 63.4 ± 5.3 mW m −2 . At the end of the MFC operation (120 h), the biofilm preferentially biodegraded EXs over acetate, removing 97.2 ± 1.9% Xs and 89.4 ± 7.5% ethylbenzene were observed, and sustained electric output was maintained. Electrochemical analyses revealed enhanced redox activity, reduced resistance to charge transfer, and increased electrochemically active surface area, which indicated that acclimation improved extracellular electron transfer. Microscopic, microbial and electrochemical characterizations confirmed the formation of a dense and conductive biofilm under EXs feeding, composed mainly by Acetobacterium , Pseudochrobactrum , and Acinetobacter , while simultaneously generating electricity, with H 2 as intermediate of electron transfer. Overall, the results demonstrate that microbial acclimation enhances electrochemical performance and alkylbenzene biodegradation, highlighting that MFCs are potential integrated platforms for remediating aromatic hydrocarbon while recovering energy.

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Cite This Study

Souza et al. (2026) studied this question.

synapsesocial.com/papers/6a095bdd7880e6d24efe1c0ahttps://doi.org/10.1002/celc.70232
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