Micrografting is a modern aseptic plant grafting technique performed in vitro , in which shoot tip explants are grafted onto sterile rootstocks to achieve physiological integration. It is widely used in plant fundamental research and horticultural applications. Recent technological advances have made this technique more powerful and versatile. This review begins with the historical background of micrografting, systematically outlines its development, provides a detailed comparison with conventional grafting in terms of success rate, cost, and technical requirements. We also highlight its advanced applications in areas including molecular transport research, graft compatibility assessment, virus eradication, conservation of rare species, and the production and rescue of novel or recalcitrant plant materials. Finally, we summarize the current status and challenges of micrografting and propose that the deeper understanding of graft formation mechanism, combined with cutting-edge approaches, will help bridge the gap between laboratory research and field applications.
Liu et al. (Fri,) studied this question.