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•Analytical performance of the Sebia free light chain ELISA was evaluated in systemic AL amyloidosis and compared with Freelite and N-Latex assays.•Sebia reported lower involved and difference FLC concentrations than comparator assays, particularly in λ-type disease.•Sebia showed reduced detection rates for abnormal κ/λ ratios compared with N-Latex and Freelite, most pronounced in λ-type disease.•Combining Sebia with serum and urine immunofixation improved detection of amyloidogenic clones.•These findings demonstrate that FLC assays are not interchangeable and require assay-specific interpretation.
Zhao et al. (2026) studied this question.