Modulating Lipid Composition in Anti-Mycobacterial Nitric Oxide-releasing Liposomes

Several liposomal systems were used to encapsulate nitric oxide (NO) donor molecules for study as a potential drug delivery system against intracellular infection. In addition to providing membrane permeability, the liposomal bilayer helps to fine tune NO release beyond the release kinetics of the encapsulated donor molecule. Four liposomal formulations with different lipid compositions were developed and characterized to examine the effects of lipid content on liposomal size, dispersity, charge, encapsulation of NO donor, and NO release. Additionally, because NO-releasing liposomes are not shelf-stable at 4°C, we report a liposomal formulation and storage protocol that allows for 86% NO retention up to 28 days. By modulating lipid composition of NO-releasing liposomes, NO delivery to sites of infection as well as storage stability may be improved, increasing this system’s utility as an antibacterial therapeutic.