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August 1, 1978Journal of Histochemistry & Cytochemistry332 citationsOpen Access

Acetylation Reduces Background in Autoradiographs of Drosophila Chromosomes

Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with 125I-labeled RNA.

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Authors

SHShizu HayashiIGI.C. GillamADA. D. Delaney

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Overview

Randomized trial shows acetylation decreases background noise in autoradiographs, enhancing chromosomal visibility.

Key Points

  • This research aims to evaluate the impact of acetylation on autoradiography results in Drosophila chromosomes.
  • Prepared chromosomes from larval salivary glands of Drosophila melanogaster were hybridized with [125I]-labeled RNA.
  • Autoradiography was conducted to assess the binding of radioactivity to chromosome regions.
  • Acetylation of chromosome preparations was performed prior to hybridization to reduce background noise.
  • Acetylation significantly decreased background radioactivity in autoradiographs, revealing clearer chromosomal labeling.
  • Without acetylation, radioactivity masked specific sites, complicating the detection of labeled chromosomes.

Cite This Study

Hayashi et al. (1978) studied this question.

synapsesocial.com/papers/6a0cc7259d761985b14a4836https://doi.org/10.1177/26.8.99471
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